Microbial transformation of some simple isoquinoline and benzylisoquinoline alkaloids and in vitro studies of their metabolites

Simple isoquinoline alkaloids (heliamine, dehydroheliamine), a phthalide alkaloid noscapine, and an aporphine boldine are biosynthetically derived from amino acid tyrosine. These substrates simple synthetic (2-acetyl-7-amino-1,2,3,4-tetrahydroisoquinoline) contain nucleus. The biotransformation of these via reduction, oxidation, hydroxylation, N-oxidation reactions with different microorganism produced nine metabolites, namely: N-(2-acetyl-1,2,3,4-tetrahydroisoquinolin-7-yl) acetamide (Metabolite 1), heliamine N-oxide 2), 6,7-dimethoxyisoquinoline 3), 3,4-dihydro-6,7-dimethoxy isoquinolin-1-one 4), 5), dehydroheliamine 6), cotarnine 7), 5-hydroxy 8), 9). Primarily, the metabolites structurally elucidated by one-dimensional (1D) two-dimensional (2D) nuclear magnetic resonance (NMR) analyses, high-resolution electrospray ionization mass spectrometry (HR-ESIMS). Furthermore, their isolated evaluated in vitro for anti-inflammatory, antimicrobial, cytotoxicity, anticancer activities. studies reveal that some compounds potential as antitumor, antimicrobial leads.